The main objective of this study is to compare the mydriatic effect of tropicamide and epinephrine in rabbits.Preliminary test was done to compare mydriatic effect of balanced salt solution epinephrine and tropicamide.Minimal dose of tropicamide with maximal mydriasis was likewise done using three dilutions.Lastly, comparison of mydriatic effect of tropicamide with epinephrine was done.Preliminary test showed comparable results between tropicamide and epinephrine, with no notable change in size of pupils using BSS.Determination of minimal dose of tropicamide was found to be 0.1 nl of original 0.5% solution diluted with 0.5 ml of BSS, showed maximal mydriasis.Tropicamide showed slower onset of 30 seconds, but onwards until 30 minutes after, it is comparable with sustained mydriasisby epinephrine.This study showed that intracameral tropicamide has comparable mydriatic effect with epinephrine in rabbits.