Korean Journal of Nuclear Medicine  1999;33(3):306-315

Studies on the Radiation Induced Apoptosis by Morphological and Biochemical Analysis in A431 Cells.

Ji Yeul KIM ; Hee Seung BOM ; Keun Hee CHOI

Keywords

Radiation-induced apoptosis; A431 cell line; Apoptotic body; DNA ladder

Country

Republic of Korea

Language

Korean

Abstract

PURPOSE: We performed this study to evaluate the process of radiation induced apoptosis in A431 skin epithelial cancer cell line. MATERIALS AND METHODS: Low to high dose radiation (0, 2, 5, 10, 25 Gy) was given to A431 cells by Cs-137 cell irradiator. Apoptosis was evaluated by cell morphology, dye exclusion test, and DNA laddering. RESULTS: Cell viability decreased as the radiation dose increased. Number of apoptotic bodies increased as radiation dose increased. It increased most significantly at 12 hours after irradiation. Lactate dehydrogenase activity in culture medium increased according to radiation dose and time after irradiation. CONCLUSION:: Radiation-induced apoptosis which was the main course of cell death in A431 cells could be analyzed quantitatively by counting apoptotic bodies under microscope. Apoptosis increased as radiation dose increased.