Journal of Forensic Medicine 2017;33(3):225-231

doi:10.3969/j.issn.1004-5619.2017.03.002

The Changes of HIF-1α and VEGF-A in Myocardial Tissue of Rats with Arrhyth-mias

Yuan ZHANG ; Zhipeng CAO ; Ruiming MAO ; Zhongbo DU ; Li MI ; Xinyi LUO ; Meihui TIAN ; Baoli ZHU

Keywords

forensic pathology; arrhythm ias; cardiac; m yocardium; hypoxia inducible factor 1; vascular endothelial grow th factor-A; rats

Country

China

Language

Chinese

Abstract

Objective T o observe the expression changes of hypoxia inducible factor-1α (H IF-1α) and vascular endothelial grow th factor-A (V E G F-A ) in rats w ith arrhythm ias, and to explore the differences of the expression pattern in the tw o indicators of acute m yocardial ischem ia caused by arrhythm ias and coronary insufficiency. Methods T he arrhythm ia w as induced by C aC l2, and the expression changes of H IF-1α and V E G F-A w ere detected by im m unohistochem istry, W estern blotting and real-tim e PC R w ithin 6 h after the arrhythm ia in rats. Results T he expression of H IF-1α and V E G F-A show ed diffuse in the m yocardial tissue of rats died from arrhythm ias. B oth of them increased in the early arrhythm ia, then decreased. E xtensive m yocardial ischem ia happened at the beginning of arrhythm ia occurrence and its range didn't expand w ith tim e. Conclusion T he expressions of H IF-1α and V E G F-A in m yocardium of the rats w ith arrhythm ia can provide evidence for the differential diagnosis of acute m yocardial is-chem ia caused by fatal arrhythm ia and coronary insufficiency.