Journal of Forensic Medicine 2017;33(3):293-300

doi:10.3969/j.issn.1004-5619.2017.03.017

Research Progress on the Detection Method of DNA Methylation and Its Applica-tion in Forensic Science

Yanchai NIE ; Lijuan YU ; Hua GUAN ; Ying ZHAO ; Haibo RONG ; Bowei JIANG ; Tao ZHANG

Keywords

forensic genetics; D N A m ethylation; review; tw ins; m onozygotic; third generation sequencing

Country

China

Language

Chinese

Abstract

A s an im portant part of epigenetic m arker, D N A m ethylation involves in the gene regulation and attracts a w ide spread attention in biological auxology, geratology and oncology fields. In forensic science, because of the relative stable, heritable, abundant, and age-related characteristics, D N A m ethyla-tion is considered to be a useful com plem ent to the classic genetic m arkers for age-prediction, tissue-identification, and m onozygotic tw ins' discrim ination. V arious m ethods for D N A m ethylation detection have been validated based on m ethylation sensitive restriction endonuclease, bisulfite m odification and m ethylation-C pG binding protein. In recent years, it is reported that the third generation sequencing m ethod can be used to detect D N A m ethylation. T his paper aim s to m ake a review on the detection m ethod of D N A m ethylation and its applications in forensic science.