Journal of China Pharmaceutical University 2017;48(2):178-183

doi:10.11665/j.issn.1000-5048.20170208

Identification of major constituents from propolis by LC-IT-TOF-MS

Jing YAO ; Jianguo SUN ; Liang WU ; Hao WANG ; Guangji WANG ; Wencai YE

Keywords

propolis; LC-IT-TOF-MS; qualitative analysis

Country

China

Language

Chinese

Abstract

A qualitative analytical method of liquid chromatography coupled with ion trap time-of-flight mass spectrometry (LC-IT-TOF/MS) was developed for identification of major constituents in propolis from China (Shandong).The LC-IT-TOF-MS was performed on a Shim-pack VP-ODS column (2.0 mm× 150 mm,5 μm) with the mobile phase consisting of acetonitrile and water containing 0.2％ formic acid in gradient mode,and the flow rate was set at 0.3 mL/min.Negative ion mode was used for IT-TOF-MS.According to the accurate molecular weight,MS fragment pathway,comparison with the retention time of reference compounds,total 31 compounds,including twenty-five phenolic acids and six flavonoids were identified.The LC-IT-TOF-MS qualitative analysis method can be used for analyzing major components of propolis quickly and accurately.