Toxoplasmosis caused by Toxoplasma gondii is one type of zoonotic, parasitic diseases seriously endangering human health. Vaccine recently becomes the highlight in control of this parasite. ROPs antigen is an effective candidate molecule of vaccine. This review outlines the status in the research of ROPs vaccine of Toxoplasma gondii mediated by bacteria such as Lactococcus lactis, Mycobacteria smegmatis, Bacille calmette-Guerin, Agrobacterium tumefaciens and Pichia Pastoris, and viruses such as Canine adenovirus type 2, Adenovirus serotype 5, Feline herpesvirus type-1 and Vaccinia virus.