Objective: To compare the inclusion rate of menthol with β-cyclodextrin (β-CD) from different sources.Methods: β-Cyclodextrin-menthol inclusion complexes were prepared by a grinding method.The inclusion compound was verified by thin layer chromatography (TLC), powder X-ray diffraction (PXRD) and gas chromatography (GC).The optimized conditions of PXRD were as follows: the graphite monochromator was Cu Kα, the voltage was 40 kV, the current was 40 mA, the 2θ scanning range was from 3° to 35°, the scanning step was 0.02° and the scanning rate was 5°·min-1.The optimized conditions of GC were as follows: an Agilent DB-wax capillary column, the column temperature was 120°, the inlet temperature was 250°, the detector temperature was 250° and the split ratio was 10∶1.Results: The results of TLC and PXRD proved the formation of inclusion complexes.The inclusion rate of menthol with β-CD from home and abroad was 25% and 47.9%, respectively.Conclusion: The method is simple, accurate and rapid, which can be used to determine the inclusion rate of β-CD from different sources.The results show that the inclusion effect of imported β-CD is better than that of domestic β-CD.