Chinese Journal of Nervous and Mental Diseases 2017;43(2):116-119

doi:10.3969/j.issn.1002-0152.2017.02.011

The study on Notch1 pathway-related tubular formation of glioma cell lines

Qingping ZHANG ; Xin MEI ; Chao KE ; Yinsheng CHEN ; Furong CHEN ; Jianliang CHEN ; Zhongping CHEN

Keywords

Notch1; Glioma; 3D cell culture; Tubular formation

Country

China

Language

Chinese

Abstract

Objective To explore the mechanism of the formation of glioma cells derived vessels (GCDV).Methods The tubular formation assay was performed on 3D cell cultures of U87,U251,U373,SF295,T98G,SKMG-4 and C6 glioma cell lines.The expression of Notch1 、Dll4 were examined by western blot analysis.Result The mean number of vasculogenic channels of cell lines C6,U373,U87,T98G,SF295,U251 and SKMG-4 per area (100×) were 25.2±5.0,36.4±3.2,19.0±2.2,12.6±2.4,4±2.2,0.2±0.4 and 0,respectively.The relative protein densities of Notchl in U87,U251,T98G,SF295,SKMG-4,C6 and U373 were 0.34,0.21,0.79,0.04,0.28,1.75 and 1.19,which were significantly related with the tubular formation ability (P＜0.05).However,the expression of Dll4 was not associated with tubular formation ability (P＞0.05).Conclusion Notch1 may play a key role in tubular formation of glioma cells while the role of Dll4 in the process needs further study.