Under the background of Internet plus,the medical work emerged some unprecedented new work characteristics,medical workers' practice environment also changed,and then made the disadvantages of the culti-vation of humanistic spirit in medical students become more and more obvious.By analyzing the status quo of medi-cal diagnosis and treatment,the current situation of the cultivation of medical students,the importance of humanis-tic spirit in the cultivation of contemporary medical students,and how to make up for the lack of humanistic spirit in the context of Internet plus,this paper laid a foundation for the transformation of the cultivation of humanistic spirit in medical universities and pointed out the measures for the cultivation of humanistic spirit of medical students un-der the background of Internet plus:endowing humanistic spirit with a new era significance,creating new commu-nication dimension of humanistic spirit,shaping new educational idea of humanistic spirit,customizing new training mode of humanistic spirit,and improving new educational power of humanistic spirit.