Objective To confirm the specificity of antibodies recently found in a hospitalized patient.Methods Methods including antibody screening,antibodies identification,alloantibody titers,isolation of red blood cells by high speed capillary centrifugation and determination of Rh phenotype were employed to investigate the characteristics of antibodies.Results Anti-C alloantibody and anti-e autoantibodies were detected in the patient.Autoantibodies were non-specific in the early stages,and gradually changed into specific auti-e antibodies.The titers of anti-C alloantibody were always lower than 4.Conclusion The identification of antibodies should be integrated as far as possible in various aspects of methodology.