Chinese Journal of Vaccines and Immunization 2008;0(06):-

Application of a Simple Method for Detection of Rubella Virus Genome by Loop-mediated Isothermal Amplification (LAMP)

Shuang WANG ; Jianhui ZHOU ; Xiang HOU

Keywords

Rubella virus; Reverse transcription loop-mediated isothermal amplification; Nucleic acid

Country

China

Language

Chinese

Abstract

Objective To use a new simple Reverse Transcription Loop-mediated isothermal amplification(RTLAMP) method was applied to detect rubella virus nucleic acid and compared with Reverse TranscriptionPolymerase Chain Reaction (RT-PCR).Method Comparing the detection rate of the RT-LAMP method with that of RT-PCR for detecting rubella virus nucleic acid from rubella virus.Results The nucleic acid positive rates of all 11 strains of rubella virus were 100% by the two methods,the positive rate was 55%.Conclusion RT-LAMP is more simple and convenient than RT-PCR.