OBJECTIVE To master the distribution and resistance of the isolates from adult urinary tract,in order to make correct treatment plan,and elvaluate the effect of treatment measures. METHODS The bacteria were isolated from meidstream urine of 441 urinary tract infection patients from Nov 11,2006 to Aug 8,2007,and the resistance of bacteria was detected. RESULTS The total number of isolated pathogenic bacteria was 100. Among them,the Gram-negative bacteria,Gram-positive bacteria and fungi were 65 (65%),22(22%) and 13 (13%),respectively. The top three of Gram-negative bacteria were Escherichia coli (41%),Enterobacter cloacae (12%),and Klebsiella pneumoniae (5%). 58.5% E. cloacae and 60% K. pneumoniae could produce extended spectrum ?-lactamases (ESBLs). The most of bacteria were multi-resistant to antibiotics,but all of isolated Gram-negative bacteria were sensitive to imipenem and 4.5% strains of Gram-positive bacteria were resistant to vancomycin. CONCLUSIONS The resistance of pathogenic bacteria is serious,so their resistance and administration of antibiotics must be monitored and controlled.