Chinese Journal of Hypertension 2006;0(09):-

p38 mitogen-activated Protein Kinase in Vascular Smooth Muscle Cells Proliferation in SHR

Liuqing XU ; Qiong YE ; Changsheng XU ; Huajun WANG ; Kegui WU

Keywords

p38 mitogen-activated protein kinase; Angiotensin Ⅱ; Platelet-derived growth factor; Cell proliferation; Vascular smooth muscle cell

Country

China

Language

Chinese

Abstract

Objective To investigate the role of p38 mitogen-activated protein kinase(p38MAPK) pathway in the proliferation of SHR vascular smooth muscle cells(VSMC) induced by angiotensin Ⅱ(Ang Ⅱ) and platelet-derived growth factor-BB(PDGF-BB).Methods VSMC were obtained from SHR thoracic aorta.DNA synthesis was quantified by measuring [3H]-thymidine incorporation.The p38MAPK activity was evaluated by immunobloting technique with phospho-p38MAPK antibody.Results 1) Ang Ⅱ(10-8-10-6 mol/L),PDGF(3-30 ng/L) dose-dependently increased the proliferation activity of VSMC([3H]-TdR incorporation).Ang Ⅱ(10-7 mol/L) or PDGF(10 ng/L) significantly increased [3H]-TdR incorporation rate in VSMCs(Ang Ⅱ:11 588?1322 vs control 2546?207 counts/min,P