International Journal of Biomedical Engineering 2012;35(2):-

doi:10.3760/cma.j.issn.1673-4181.2012.02.007

Study of photosensitizers absorbed process by cells based on image processing technology

Zhan SHU ; Yingxin LI ; Jichun YANG ; Hongli CHEN ; Xiaoxi DONG

Keywords

Image processing; Matlab; Photosensitizer; Fluorescence intensity

Country

China

Language

Chinese

Abstract

ObjectiveTo study the feasibility of analyzing photosensitizers absorbing process by cells based on image processing technology.MethodsFluorescence images of Laryngeal cells were taken by inverted fluorescence microscope after adding photosensitizers for a certain time.Parameter L that reflects the fluorescence intensity of cells in different times was obtained and the calculation results were compared.ResultsThe fluorescence intensity of cells increased over time.Sobel operater and Otsu algorithm can both reflect the fluorescence intensity of images.ConclusionImage processing technology can effectively analyzes the process of photosensitizers absorbed by cells.