Chinese Journal of Nosocomiology 2006;0(12):-

Fluorescent Antibody Method for Detection of Helicobacter pylori in Dental Plaque and Gastric Fluid: An Evaluation

Shiying LI ; Haipu ZHANG ; Xianling WANG ; Xiaoming ZHAO ; Dongqi GAO ; Tinggui WANG

Keywords

Helicobacter pylori; Fluorescent antibody; Dental plaque; Gastric fluid

Country

China

Language

Chinese

Abstract

OBJECTIVE To screen for a simple,accurate and no-traumatic detecting method of Helicobacter pylori(Hp). METHODS Hp in gastric fluid and dental plaque was detected with fluorescent antibody method,bacterial culture,urease test and Hp diagnosis card at the same time in 62 cases with gastric and duodenal disease.The gather of gastric fluid applied the capsule method. RESULTS The detective rates of Hp in gastric fluid by four methods were 85.5%,9.7%,61.3%,and 56.5%;the detective rates of Hp in dental plaque by four methods were 88.7%,25.8%,69.4%,and 90.3%,respectively. CONCLUSIONS Fluorescent antibody method combined with capsule method detecting Hp in gastric fluid is specific,sensitive and without trauma.Thus,it is suggested to be used clinically.