OBJECTIVE To investigate the nonfermenter distribution and drug-resistance in the clinical specimens,and instruct clinical application of antibiotics reasonably. METHODS Bacteria were cultured,isolated and identified with ATB Expression microbe identification system.Drug-resistance was detected with ATB PSE and statistically analyzed. RESULTS A total of 1 106 strains of bacteria were isolated from 7 395 specimens,the isolation rate was 14.9%.The isolation rate of Pseudomonas aeruginosa was the highest.The isolation rate of nonfermenter was the highest from sputum specimens.Nonfermenter was the most sensitive to imipenem except for Stenotrophomonas maltophilia and Chryseobacterium meningosepticum,after imipenem was cefoperazone/sulbactam. CONCLUSIONS The nonfermenter isolated from different clinical specimens and its drug-resistance are variant.To reduce the production of drug-resistance and control the nosocomical infection,the specimens must be collected and detected timely,the antibiotics are selected according to the results of susceptibility test.