Tuberculosis and Respiratory Diseases  2006;60(5):554-563

doi:10.4046/trd.2006.60.5.554

The Effects of Air-borne Particulate Matters on the Alveolar Macrophages for the TNF-alpha and IL-1beta Secretion.

Tian Zhu LI 1 ; Soo Jin LEE ; Se Jong PARK ; Byung Joon CHANG ; Jong Hwan LEE ; Kil Soo KIM ; Myoung Heon LEE ; Nong Hoon CHOE

Affiliations

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Keywords

Particulate matter (PM); Alveolar macrophages; Tumor necrosis factor-alpha (TNF-alpha); Interleukin 1beta (IL-1beta)

Country

Republic of Korea

Language

Korean

Abstract

BACKGROUND: PM is known to induce various pulmonary diseases, including asthma, cancer, fibrosis and chronic bronchitis. Despite the epidemiological evidence the pathogenesis of PM-related pulmonary diseases is unclear. METHODS: This study examined the effects of PM exposure on the secretion of TNF-alpha and IL-1beta in the cultured alveolar macrophages. The cultured primary alveolar macrophages were treated with the medium, PM (5~20 microgram/cm2), LPS (5ng/ml), and PM with LPS for 24h and 48h respectively. ELISA was used to assay the secreted TNF-alpha and IL-beta in the culture medium. Western blotting was used to identify and determine the level of proteins isolated from the culture cells. The cells cultured in the Lab-Tek(R) chamber slides were stained with immunocytochemical stains. RESULTS: PM induced TNF-alpha and IL-1beta secretion in the culturing alveolar macrophages, collected from the SPF and inflammatory rats. However, the effects were only dose-dependent in the inflammatory macrophages. When the cells were co-treated with PM and LPS, there was a significant synergistic effect compared with the LPS in the both cell types. CONCLUSION: PM might be play an important role in the induction and/or potentiation of various lung diseases by oversecretion of TNF-alpha and IL-1beta.