Breast cancer is one of the most common cancers in women. Current clinical prognostic indicators and practice guide-lines show that almost 70% of node-negative and 30% of lymph node-positive breast cancer patients can survive 5-10 years without dis-tant metastasis after surgery. Patients also unnecessarily suffer the side effects of chemotherapy. Assays on multi-gene expression levels as prognosis prediction indicators have been successfully used to predict the prognosis of breast cancer patients because of the abnor-mal expression levels of a series of metastasis-related genes in cancer cells with high metastatic potential. In this review, we highlight the progress of research on predicting breast cancer prognosis based on multi-gene expression levels detected by microarray and qRT-PCR methods.