For the purpose of opening up the future of more widespread application of cord blood,and also for the purpose of setting up the, bank of hemopoietic stem sells in cord blood,an effective method of cryopreserving the cord blood was approached, indicating, that it was appropriate that the time of cryopreserving the hemopoietic stem cells in cord blood at 4℃ should be 2-3 days (the storage of hemopoietic stem cells in cord blood with liquid nitrogen was an effective method of cryopreserving the hemopoietic stem cells in cord blood,with high recovery rate,and longterm cryopreservation without any change in the recovery rate;the effect of storing the hemopoietic stem cells in freezer at-80℃ was similar to that of cryopreserving them with liquid nitrogen,and the former was simple,e-conomic, convenient and practical, and needed no any programmed temperature-dropping apparatus .At the same time, it is convenient to using the stem cells in large scale and to setting up the bank of them. Preserving the whole cord blood can avoid the loss of hemopoietic stem cells in cord blood during the centrifuging process,with the effect of preservation being unchanged.