Chinese Journal of Cancer Biotherapy 1994;0(01):-

Application of MTT Colorimetric Assay in Research of Cytokine Activity and Cytotoxicity of Killer Cells

Zhigang TIAN ; Jianhua ZHANG

Keywords

MTT colorimetric assay; cellular cytotoxicity; cytokines; lymphocyte proliferation; tumor cell chemosensitivity

Country

China

Language

Chinese

Abstract

We used MTT assay to test the cellular cytotoxicity ( NK, LAK, CTL, Macrophage), cytokine activities ( 1L-1, 1L-2, 1L-6, TNF), proliferation of lymphocytes and chemosensitivity of tumor cells, and compared it with radioactive isotope assay. The results showed that the MTT assay may be used to test the cellular cytotoxicity, cytokine ac-tivity, proliferation of lymphocytes and chemosensitivity of tumor cells. We think it is a simple, rapid, economic and safety method.