PURPOSE: The purpose of this study was to evaluate changes regarding knowledge, attitude and practice toward end-of-life care in nursing university students. METHOD: This study adopted nonequivalent control group pretest-posttest design. The subjects consisted of 35 in the experimental group and 35 in the control group. The education was performed for 2 hours a session, once a week for 16 weeks. Data was analyzed by the SPSS/WIN 14 computer program, and included chi2 test, independent t-test, and repeated measure ANOVA. RESULTS: There were statistically significant differences in knowledge toward end-of-life care between the experimental group and the control group. CONCLUSION: Findings suggested that holistic education on end-of-life care was effective to change students' knowledge toward end-of-life care. Therefore, holistic education is recommended as nursing education for nursing university students.