AIM: To determine the content of(+)-catechin and (-)-epicatechin and berberine hydrochloride in Wangying Capsule(Rhizoma Picrorhizae,Rhizoma Coptidis,Catechu,etc.). METHODS: A RP-HPLC method was established.The chromatographic column was Diamonsil-C_(18).The mobile phase was 0.04 mol/L citric acid-N,N-dimethylformamide-tetrahydrofuran(40∶8∶2) for determining(+)-catechin and(-)-epicatechin with the flow rate of 1.0 mL/min,and the detection wavelength of 280 nm.The mobile phase was acetonitrile-water(52∶48)(including 0.34% potassium dihydrogen phosphate and 0.17% sodium dodecyl sulfate) for determining berberine hydrochloride with the flow rate of 1.0 mL/min,and the detection wavelength of 265 nm. RESULTS: The average recoveries for(+)-catechin and(-)-epicatechin and berberine hydrochloride were 100.9% and 99.9% and 97.0%,precision of the method were 2.52% and 2.53% and 2.70%(RSD,n=6),respectively. CONCLUSION: The method can be used for quantitative determination of the preparation.