Objective To investigate the relation between non-alcoholic fatty liver disease (NAFLD) and total cholesterol (TC),triglyceride (TG),low density lipoprotein-cholesterol (LDL-C) in blood,and provide reference for clinical diagnosis and treatment.Methods Eighty cases of normal young men without NAFLD were as control group,80 cases of NAFLD without type 2 diabetes mellitus were as group A,and 80 cases of NAFLD combined with type 2 diabetes mellitus were as group B.Then the blood lipid was analyzed and compared among the 3 groups.Results The TC,TG and LDL-C levels in control group were (4.09 ± 1.01),(1.94 ±2.14) and (2.47 ±0.93) mmol/L respectively,in group A were (5.16 ±2.13),(3.01 ± 1.56) and (3.15 ± 1.12) mmol/L respectively,and in group B were (6.21 ± 1.17),(4.35 ± 1.20)and (3.21 ± 0.15) mmol/L respectively,there were statistical differences among the 3 groups (P ＜ 0.05).The TC,TG and LDL-C levels in group A and group B were significantly higher than those in control group,in group B were significantly higher than those in group A,and there were statistical differences (P ＜ 0.05).Conclusions NAFLD and blood lipids is closely related,and it affects normal operation function of part lipid metabolism.Patients with type 2 diabetes mellitus are significantly abnormal in lipid metabolism.