Journal of Bacteriology and Virology  2005;35(3):209-216

A Bacterial Flagellin, Vibrio vulnificus FlaB, Induces Human Dendritic Cell Maturation.

Byung Chul JEONG 1 ; Soo Young KIM ; Bo Hwa CHOI ; Myong Suk PARK ; Je Jung LEE ; Joon Haeng RHEE ; Shee Eun LEE

Affiliations

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Keywords

Flagellin; TLR5; Vibrio vulnificus; Dendritic cell; Flow cytometry

Country

Republic of Korea

Language

English

Abstract

The motile marine bacterium, Vibrio vulnificus has a total of six flagellins. Flagellin is a structural component of flagellar filament in various locomotive bacteria and is the ligand of Toll-like receptor 5 (TLR5). TLRs, highly expressed on various types of cells including dendritic cells (DCs), recognize invading microorganisms and finally trigger host immune responses. In this study, we prepared all of six recombinant flagellin proteins and assessed the effect of six flagellins on IL-8 activation through TLR5 recognition. Although showed different activities, five out of the six flagellins stimulated significant IL-8 activation. We also investigated the immunomodulatory roles of Vv-FlaB, the crucial building block of V. vulnificus flagellar filament, on human dendritic cells. Treatment of immature DCs with Vv-FlaB resulted in an increased expression of co-stimulatory molecules and induced strong allo-T cell proliferative activities of the DCs. These results show that the Vv-FlaB may serve an epochal immune adjuvant possessing effective immunomodulatory activities.