To construct human tissue inhibitor of metalloproteinase-2 and express it in P.pastoris.The sequence was designed according to the amino acid sequence of the TIMP-2 and high-level expression sequences of P.pastoris.We gained the TIMP-2 gene by artificially synthesis method.The gene was cloned into pPIC9 to construct a recombinant vector pPIC9-T2.The vector was transformed into P.pastoris by electroporation,through phenotype selection and induced with methanol we get the expression cell.Separation,purification and bioactivity analysis of the expressed products was performed.