Objective:To analyze the role of human mda-7/IL-24 gene on the proliferation of human non-small cell lung cancer cell line (H460). Methods:The mda-7/IL-24 cDNA was subcloned into expression vector pcDNA3 and transient transfected in human lung carcinoma cell H460. The overexpressed mda-7/IL-24 was identified by RT-PCR using SP6 primer and mda-7/IL-24 upstream primer. The role of human mda-7/IL-24 on the proliferation of H460 cells was analyzed with MTT after transfection of (pcDNA3)-mda-7/IL-24 for 72h. Results:The mda-7/IL-24 cDNA was subcloned into pcDNA3 successfully. RT-PCR study showed that mda-7/IL-24 could express in H460 cells at different ratio of DNA and lipofectamine (1 ?g ∶ 2?l-1?g ∶ 4?l). Compared with H460 cells and H460 cells transfected with (pcDNA3), pcDNA3-mda -7/IL-24 could inhibit the proliferation of H460 cells. The inhibition rate was (18.4%). Conclusion:The mda-7/IL-24 can inhibit the proliferation of human non-small-cell lung carcinoma cell H460.