OBJECTIVE To investigate multidrug-resistant bacteria infection in newborn department,for rationally use of antibiotic. METHODS A total of 1927 patients in newborn department scientific were investigated for their multidrug-resistant bacteria infection. RESULTS There were no statistical differences of multidrug resistance bacteria infection in the four seasons. From 1927 newborn patients,128 patients were with multidrug-resistant bacteria infection,the positive rate was 6.64%,83.59% origin of Society area,in 128 patients children of multidrug resistance bacteria infection,happen infection were 134 example 86.57% were respiratory tract infection,70.83% resistance bacteria including Klebsiella pulmonary,appear multidrug resistance bacteria infection previous 62.28% were use antibiotic. CONCLUSIONS Newborn deartment The multidrug resistance bacteria infection is related to occurved in newborn department abuse and unrationaly use of antibiotics. Its should be strengthened to supervise and manage.