Immunological Journal 2001;(2):105-106,109
doi:10.3969/j.issn.1000-8861.2001.02.008
Expression of the extracellular domain of human CD7 in Escherichia coli
Yanchun ZHOU ; Xihua ZHU ; Yunhui HUANG
Country
China
Language
Chinese
Abstract
Objective To get the antigen of CD7.Methods The extracellular domain of human CD7 was cloned from a plasmid containing the full length of human CD7 cDNA and expressed in pinpoint-xa3 prokaryotic system. Results Analysis with SDS-PAGE and Western-blotting showed that the expressed protein could bind to the anti-CD7 mAb specifically and is about 30 000 u in molecular weight. Conclusion These results paved the way for preparing anti-CD7 engineering antibody.
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