Five kinds of probiotic bacteria are immobilized and cultured continuously in simulated intestinal systems with corn fiber as the carrier.The biomass and the colonization in biofilm are investigated by selective culture methods and scanning electron microscope.Bifidobacteriurn longum TQ21-2-2,Lactobacillus acidophile CICC06005,Clostridum butyric TO-A,Bacillus mesentericus TO-A and Enterococcus faecalis T-110 can coexist stably.The biomass of five probiotics in the immobilized phase is greater than that of liquid in the continuous shake flask reactor.Five kinds of probiotic bacteria formed biofilm on corn fiber which be observed by SEM.Continuous culture of immobilized cells could be used better model than that of liquid, the model can be used to study for microecology and microecologocal agent.