OBJECTIVE To apply the comprehensive interventional measures to manage the clinical application of antibacterials and provide a scientific basis for their management.METHODS Since 2004 we strengthened the propagend a about the hospital infection knowledge worked out the quantification standards about the antibacterial use rate and cost ratio,the interventional measures about the rational use of antibacterials,and made an investigation about antibacterial use rate and cost ratio before and after intervention.RESULTS It indicated the antibacterial use rates before and after intervention and among operated patients had the statistically significant differences.CONCLUSIONS The application of comprehensive interventional measures to manage clinical use of antibacterials could decrease the antibacterial use rate and increase the self-consciousness among clinicians in rational use of antibacterials.