Chinese Traditional Patent Medicine 1992;0(02):-

HPLC Determination of Active Constituents in Xiaoerniuhuang Powder

Lin SHI ; Hongjian YU

Keywords

HPLC Determination; Xiaoerniuhuang Powder; berberine; berbericinine; emodin; chlorogenic acid

Country

China

Language

Chinese

Abstract

The reversed-phase high-performance chromatography was developed for determing the content of berberine, palmatine, emodin and chlorogenic acid in Xiaoerniuhuang Powder on ODS column. The powder samples extracted with methanol were determined separatly at their particular absorption points using external standard method.This method had advantages of perfect separatin, high sensitivity, good reproducibility and simple operation.