In the experiments, various media were used to isolate Campylobacter Pyloridis. Onto every medium serum, blood, vitamin, glucose, charcoal and starch were added separately. The results were compared with direct Gram's stain method and urease test. Better growth was obtained on the brain-heart blood agar; almost no. growth occured on the serum agar, normal blood agar and C. jejuni agar. The positive rate was increased after charcoal and starch were added. The positive rate was significantly increased after vitamin and glucose were added. The colonies were increased significantly. It was similar with the results of the direct Gram's stain method