OBJECTIVE: To extend clinical application scope of ketoconazole, and satisfy the needs of ophthalmologists with mycotic infection. METHODS: Polysorbate 80 was used as solubilization, acidic water dissolent, basic water pH regulator, anhydrous sodium bisufite stabilizer, and ethylparaben preservative. The eye drops were prepared according to the ordinary method of manufacturing. RESULTS: The eye drops have good stability without stimulation to eye. Its quality can by controlled for good curative effect. CONCLUSION: Ketoconazole could be prepared eye drops for clinical application.