OBJECTIVE To investigate distribution and drug resistance status of infections in urinary system and offer scientific basis for reasonable usage of antibiotics. METHODS Totally 258 isolates collected from urinary infection specimens were identified and drug susceptibility test was performed. RESULTS Escherichia coli rated the top one,73.3%.The isolates were susceptible to imipenem and amikacin.The resistant rate to other 6 antibiotics were above 50%. CONCLUSIONS The drug resistance rate of isolates collected from urinary infection specimens goes upward,so we should pay attention to.