A rapid, simple and sensitive method was establised for the evaluatiou of Chinese traditional medicine "Joufenaan" by HPLC. Sample of "Joufensan" was extracted by solvent, and the extraction analysed by HPLC using an inertsil ODS column with a solvent system, acetonitrile-water-phospboric acid-sodium dodecyl sulfate(38:62:0.1:0.5) at 40℃ and UV detector. The new method was reliable for the evaluation of ephedrine aud strychnine in "Joufensan".