Acta Nutrimenta Sinica 1956;0(03):-

EFFECTS OF SELENIUM ON GENE EXPRESSION OF PHOSPHOLIPASE D GENE IN LIVER IN DIABETIC RATS

Yuntang WU ; Zhong SUN ; Xia WANG ; Xiaoyong LIU ; Zhuangzhi ZHOU ; Yongming WANG

Keywords

selenium; diabetes; gene expression; RT-PCR; PLD

Country

China

Language

Chinese

Abstract

Objective: To study the regulation of selenium on gene expression of phospholipase D (PLD) gene in relation to liver metabolism of diabetic (DM) rats. Method: Differential display cDNA fragments were isolated from former research project in diabetic rats which were supplemented orally with 50 g/kg bw d selenium for 60d, and were cloned, sequenced, and the homology was analyzed. RT-PCR was made using the primers designed according to the sequences of cDNA fragments. Results: In differential display cDNA fragment, Se-4 and PLD were homologous with sequence identities of 100%.The expression levels of PLD mRNA in DM group and DM+Se group was higher obviously than that of NC group(P