Chinese Journal of Clinical Pharmacology and Therapeutics 2002;0(06):-

Effect of levcromakalim on ET-1 induced proliferation and expression of protein kinase C in vascular smooth muscle cells

Wenxin HUANG ; Biwen MO ; Yaozhong YANG ; Chunsheng FANG ; Zhonghua XIA ; Dihua PAN ; Tongtong XU ; Xinling MO

Keywords

levcromakalim; vascular smooth muscle cells; proliferation; protein kinase C

Country

China

Language

Chinese

Abstract

AIM:To observe the effect of levcromakalim(Lev) on endthelin-1(ET-1) induced proliferation and the expression of protein kinase C(PKC) in cultured rat vascular smooth muscle cells(VSMCs).METHODS: VSMCs were isolated from rat aorta and were cultured with different concentrations of Lev in vitro after stimulated to proliferation by ET-1(10~(-8)(mol?L~(-1))).Vitality of VSMCs was detected by MTT;DNA replication was evaluated by 3H-TdR method.Flow cytometry was used to analyze cell cycle and apoptosis.The expression of PKC? protein and mRNA were determined by Western blot and reverse transcription-polymerase chain reaction(RT-PCR).RESULTS:Vitality of VSMCs and 3H-TdR intermingle rate were decreased(P