Chinese Journal of Practical Internal Medicine 2000;0(12):-

Effects of rosiglitazone on the mRNA expressions of MMP-1、TIMP-1 and Collagen Ⅲ in rat kidney fibroblasts cultured in high glucose.

Zhaohui ZHENG ; Haijian LI ; Zhangsuo LIU

Keywords

Fibroblasts; Matrix metalloproteinases; Collagen Ⅲ; Rosiglitazone

Country

China

Language

Chinese

Abstract

Objective To investigate into the protective effects of rosiglitazone on rat kidney fibroblasts(NRK)damaged by high glucose.Methods From Jan.2005 to Sep.2005,the NRK cells were cultured in vitro,and were divided into five groups:normal glucose group(NG,1 000 mg/L D-glucose),high glucose group(HG,4 500 mg/L D-glucose),HG+RGZ(5 ?mol/L),HG+RGZ(10 ?mol/L)and HG+RGZ(15 ?mol/L).The mRNA expressions of T MMP-1、TIMP-1 and Collagen Ⅲ were measured with RT-PCR.Results Compared with NG group,the mRNA expression of MMP-1 decreased markedly in HG group(P