Journal of Pharmaceutical Analysis 2010;22(4):260-264

Inhibitory action of docetaxel on the proliferation of HeLa and SiHa cells

Yuliang ZOU ; Jun FENG ; Wenli GOU ; Meili PEI ; Fenling FAN ; Zhongming ZHANG

Keywords

docetaxel; chemotherapy; cervical cancer; HeLa; SiHa; proliferation

Country

China

Language

Chinese

Abstract

Objective To study the inhibitory action of docetaxel (DOC) on the proliferation of HeLa and SiHa cells. Methods Cell morphological changes were observed with inverted phase contrast microscope. MTT was adopted to test and calculate the cell inhibition ratio. Flow cytometry was used to detect cell cycle. Results DOC had an obvious concentration-dependent inhibitory effect on the proliferation of both HeLa and SiHa cells. The inhibition ratio of DOC on SiHa was significantly higher than that on HeLa (P<0.05). DOC blocked HeLa at G2/M phase. Under the effect of DOC, the cell cycle of SiHa was not changed much. Conclusion DOC has an obvious inhibitory action on both HeLa and SiHa cells, which shows a promising prospect of DOC in clinical treatment of cervical cancer.