Chinese Journal of Blood Transfusion 1988;0(03):-

Preparation of Protein C Deficient Plasma

Xiaopu XIAO

Keywords

PC; PCDP; McAb; Affinity chromatographic column

Country

China

Language

Chinese

Abstract

A specific monoclonal antibody(McAb),which could identfy different conformations of protein C(PC) in the presence or absence of Ca24+ .was selected and coupled with Sepharose-4B gel to form an affinity chromatographic column. When fresh plasma passed through this column.its PC would he adsorbed exclusively,and the obtained effuent was PC-deficient plasma (PCDP). The residual PC in the PCDP gained was