Objective To develop a high performance liquid chromatography method for the determination of methylisothiazolinones and chloro-methylisothiazolinoe in cosmetics. Methods The cosmetic samples were extracted with methanol. The analytes were separated with the mobile phase of V(Methanol)∶V(Water)= 25∶75 at the flow rate of 1.0 ml/min and detected at the wavelength of 276 nm. Results The linear range of the method was 0.05-100 mg/ml for methylisothiazolinone and 0.0125-25 mg/ml for chloro-methylisothiazolinoe. The limit of detection of methylisothiazolinone and chloro-methylisothiazolinoe were 0.08 and 0.2 ?g respectively. The recovery rates were 84.5%-93.9% with the RSD of 2.5%-9.4% (n=6) for methylisothiazolinone and 88.3%-92.9% with the RSD of 2.9%-5.8% (n=6) for chloro-methylisothiazolinoe. Conclusion The method is simple,rapid,accurate and is suitable for the determination of methylisothiazolinone and chloro-methylisothiazolinoe in cosmetics.