International Journal of Oral Biology  2017;42(2):39-45

doi:10.11620/IJOB.2017.42.2.039

Effect of Metformin on Cell Growth and Differentiation in Cultured Odontoblasts.

Chang Young OH 1 ; Su Gwan KIM ; Dae San GO ; Sun Kyoung YU ; Tae Hoon KIM ; Chun Sung KIM ; Joo Cheol PARK ; Do Kyung KIM

Affiliations

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Keywords

metformin; odontoblasts; differentiation; mineralization

Country

Republic of Korea

Language

English

Abstract

Metformin (1,1-dimethylbiguanide hydrochloride), derived from French lilac (Galega officinalis), is a first-line anti-diabetic drug prescribed for patients with type 2 diabetes. However, the role of metformin in odontoblastic cell differentiation is still unclear. This study therefore undertook to examine the effect of metformin on regulating odontoblast differentiation in MDPC-23 mouse odontoblastic cells derived from mouse dental papilla cells. As compared to controls, metformin significantly accelerated the mineralization, significantly increased and accelerated the expressions of ALP and Col I mRNAs, and significantly increased the accelerated expressions of DSPP and DMP-1 mRNAs, during differentiation of MDPC-23 cells. There was no alteration in cell proliferation of MDPC-23 cells, on exposure to metformin. These results suggest that the effect of metformin on MDPC-23 mouse odontoblastic cells derived from mouse dental papilla cells, facilitates the odontoblast differentiation and mineralization, without altering the cell proliferation.