Objective:To investigate the changes of Th1/Th2 ratio in the PBMC and hepatocellular carcinoma tissues of patients with heptocellular carcinoma and its clinical significance.Methods:Th1/Th2 subsets and cellular factor were detected by ELISPOT and double-sandwich ELISA.Results:Th1/Th2 ratio in the PBMC and hepatocellular carcinoma tissues of patients with heptocellular carcinoma,Th1 sebset expressing cellular factor IFN-?,IL-2,IL-12 were significantly lower,and Th2 sebset expressing cellular factor IL-4,IL-10 were significantly higher than those of the normal control group.PMBC in patients with heptocellular carcinoma producing Th1 sebset cytokins deduced by PHA and IL-12 were significantly higher and producing Th2 sebset cytokins were significantly lower than those in the three month after operation.Conclusion:Th1/Th2 ratio in the PBMC and hepatocellular carcinoma tissues were of imbalance and immune functional status of patients with hepatocellular carcinoma was inhibitory.Therefore,it was nessassory to enhance immune functional status of patients during treatment.