The detecting of HCV-RNA and genotyping of HCV in sera of 65 paid blood donors which are positive for anti-HCV were conducted,indicating that 60 of them appeared positive for HCV-RNA,and their genotype of HCV was classified as genotype- I . It was suggested that the genotype- I of HCV could be considered as the predominant infectious strain in this group of paid blood donors. It was found that the endonuclease digestion method of typing based on HCV 5' -noncoding region PCR was better compared with the direct method of typing based on HCV core region PCR in terms of its senstivity,economy and simplicity.