Chinese Journal of Endocrinology and Metabolism 1985;0(02):-

Effect of estrogen on experimental autoimmune thyroiditis in Wistar rats

Xiaoqin GUO ; Enjiang TIAN ; Jiali ZHANG ; Shujun ZHAO ; Jinlong WANG

Keywords

Thyroiditis, autoimmune; Estradiol; Interferon-? (Interferon type Ⅱ)

Country

China

Language

Chinese

Abstract

Objective To observe the effects of estradiol-17? (E 2-17?) on the induction of experimental autoimmune thyroiditis (EAT). Methods Ten-week old female Wistar rats were ovariectomized (OVX). EAT of Wistar rats was induced by immunization with thyroglobulin (Tg) from SD rats in the presence of complete Freund′s adjuvant. Thyroiditis was pathologically evaluated by HE staining. E 2-17? and PRL levels were measured by RIA, anti-Tg antibody (TgAb) was detected by ELISA, and interferon (IFN)-?, interleukin (IL)-10 mRNA in thyroid were assayed by RT-PCR. Results The serum level of E 2-17? (17.60?1.02)pmol/L and PRL (2.45?0.15)?g/L were much lower in OVX rats than intact rats 〔(58.56?6.99)pmol/L and (3.22?0.17)?g/L, respectively〕 (both P