OBJECTIVE To prevent and control nosocomial infection in clinical laboratory of health center in small towns.METHODS Hidden danger of nosocomial infection was investigated and analyzed to formulate the strategy of precaution and control.RESULTS Management status in laboratory was not standardized and occurred several administration shortages such as supervising system,precaution consciousness,operating instruction,contamination zonation,medical garbage disposal,etc.CONCLUSIONS Adopting comprehensive management,establishing relevant rules and carrying on standardized administration are essential for prevention and control of nosocomial infections in laboratory of health center in small towns.