Immunohistochemical PAP method was used to detect the fibronectin (FN) in the rat's skin incised wounds. The results demonstrated that FN manifest itself in the wall of the wounds after 15 min injury, within 1 to 8 hrs after injury, FN accumulated numerously, forming a gradient deposition. In contrast, no FN was found in wounds formed 5 min after death. The research had eatablished a new method for differentiation timing of early wound, and for differentiation of antemortom from postmortem wounds