Journal of Kunming Medical University 1989;0(01):-

Synchronous Determination of Creatine Compounds, Adenine Nucleotides, Hypoxanthine and Inosine with HPLC

Yuechun ZHU

Keywords

HPLC; Creatine compounds; Inosine Adenine nucleotides; Hypoxanthine

Country

China

Language

Chinese

Abstract

Synchronous determination of creatine Compounds, adenine nucleotides, hypoxanthine and inosine with reversed—phase HPLC was studied. The distinct separation was achieved within 10 minutes at 210nm using radial—PAK C_(18) Cartridge column under an isocratic elution of 220mmol/L K_2HPO_4/KH_2PO_4, mmol/L TBA and 13% methanol. This method proved to be reproducible, simple and accurate.