Chinese Journal of Forensic Medicine 1988;0(04):-

EXAMINATING THE SPERM ANTIGEN IN SEMINAL FLUID AND SEMINAL STAIN USING ELISA METHOD

Yaqing DUAN ; Jinghua XU ; Xiaofeng WANG ; Guanshi ZHANG ; Yuxin LI

Keywords

Monoclonal antibody against human sperm; ELISA sperm antigen; Seminal fluid; Seminal stain

Country

China

Language

Chinese

Abstract

The sperm antigens were examined using the method of ELISA and specific monoclonal antiboby against human sperm.The results of ELISA method of 10 fresh seminal fluid samples and 15 seminal stains showed 100% positive rate. Even diluted the fresh seminal fluid to 10~6 times of seminal stains extract to 51?0~5 times,both were positive also.However,it was negtive for testing other specimens such as saliva stains,urine stains,colostrum stains,sweat stains, vaginal secretion stains and human seminal stains after vesoligation. The result certificate that using specific antibody to the sperm,the ELISA method is high sensitive and specific for the sperm antigen assay.