Chinese Journal of Forensic Medicine 1986;0(02):-

ISOLATION AND PURIFICATION DF Gc PROTEIN FROM HUMAN PLASMA

Yiping HOU ; Meiyun WU

Keywords

Group-specific component (Gc) Vitamin D binding protein Serum proteins Isolation and purification

Country

China

Language

Chinese

Abstract

Group-specific component(Gc), one of the polymorphic proteins of human plasma,has been isolated and purified from human plasma of Gc 2-2 phenotype by a procedure including DEAE-Sephadex-A50,Sephadex G100 and DEAE-Sephadex-A50 chromatography. The purified Gc identified by PAGE,SDS-PAGE and immunoelectrophoresis was homogeneous and reacted specifically with the commercial anti-Gc serum (DAKO) kit. The molecular weight of the purified Gc was about 58 kd.